Phos-tag SDS-PAGE データ集 SRF
toppage

SRF

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Mw; 52,000

 

泳動結果

泳動結果

HEK293 細胞を,Ser/Thr phosphatase 阻害剤のcalyculin A,またはTyr phosphatase 阻害剤のpervanadateで処理した後,SRFのリン酸化状態の変化を
Zn2+-Phos-tag SDS-PAGEで調べています。

 

Reference なし
ゲルの条件

Zn2+ -Phos-tag: 25µM

Polyacrylamide: 5%(w/v)
    +
0.5% agarose

検出方法 WB: anti-SRF

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